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BRAND UV-Cuvette UV-Transparent Spectrophotometry Cuvettes FAQ
Browse through some common questions about the BRAND UV-Cuvette UV-Transparent Spectrophotometry Cuvettes:
| 1. | | What is the maximum operating temperature for the UV-Cuvettes? | |
| The UV-Cuvettes can be used at temperatures up to 60 degrees Celsius for several hours. |
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| | 2. | | I have selected the correct size UV-Cuvettes for my spectrophotometer, but still am not getting appropriate results. Why? | |
If you are using the appropriate window height (z-dimension) ultra-micro UV-Cuvette for your spectrophotometer, and are not getting appropriate results, it is likely due to one of two different problems.
- Extremely dilute samples.
Because the proprietary plastic has a higher absorbance than quartz, extremely dilute samples may not differ substantially from background, so you may have no other choice than to use a quartz cuvette.
- Light beam width.
Some spectrophotomers have abnormally wide light beams that can be refracted by the side walls of the cuvette. When using ultra-micro sized samples, these spectrophotometers require the use of black-masked quartz cuvettes to overcome this deficiency. |
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| | 3. | | What is the difference between your PS, PMMA and UV-Cuvettes | |
| Polystyrene (PS) cuvettes are the least expensive, however they have the lowest chemical resistance, and are only suitable for visible light applications, with a lower wavelength limit of around 340nm. Polymethyl methacrylate (PMMA or “acrylic”) cuvettes extend that range to about 300nm, or slightly into the near UV range, and have slightly greater chemical compatibility. BRAND UV-Cuvettes use a proprietary plastic to allow measurement as low as 220nm, and has resistance to many polar organics as well as many acids and bases. |
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| | 4. | | What does “mold cavity matched” mean? | |
| Cuvettes are injection molded plastic. No matter how close the tolerances are in the molds, there will be some variance. BRAND cuvettes are sorted during the manufacturing process so that each package of cuvettes contains only cuvettes made from the same mold, minimizing the cuvette-to-cuvette variance. |
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| | 5. | | Do you sell four-clear-side flourimetry cuvettes? | |
| No. |
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| | 6. | | My results with your UV-Cuvettes are different than with quartz. What’s happening? | |
| While our UV-Cuvettes allow enough light to pass to perform accurate spectrophotometry down to 220nm, they are not as transparent as quartz. When using UV-Cuvettes, any reference measurements should also be done with UV-Cuvettes. |
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Can't Find the Answer You're Looking For?If you're having trouble finding the information you want, BrandTech Customer Service is here to help. Call us at 888-522-2726, or if you prefer, contact us online.
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